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[Effects of embryonal bone tissue on hemopoiesis]
Vestnik Akademii Meditsinskikh Nauk SSSR
|January 1, 1990
Summary
Human fetal bone (FB) supports bone marrow cell viability and inhibits fibrous tissue growth in vitro. FB transplantation also improved survival and recovery from chemotherapy in rats.
Area of Science:
- Biomedical research
- Cell biology
- Hematology
Background:
- Bone marrow trephine biopsy samples are crucial for assessing hemopoiesis.
- Understanding stromal cell influence on hemopoietic cells is vital for regenerative medicine.
- Fetal bone's potential role in supporting bone marrow recovery is largely unexplored.
Purpose of the Study:
- To investigate the influence of human fetal bone (FB) on bone marrow hemopoietic and stromal cells.
- To evaluate the effect of FB on hemopoiesis and survival in a rat model post-chemotherapy.
Main Methods:
- Morphometric assay of bone marrow trephine biopsy samples cultivated using the Marbrook system (1-5 weeks).
- FB transplantation into rats previously administered lethal and sublethal doses of cyclophosphamide.
- Assessment of hemopoietic cell viability, fibrous tissue proliferation, and animal survival rates.
Main Results:
- Long-term cultivation of bone marrow samples showed increased fibrous tissue and loss of hemopoietic elements.
- Combined cultivation of bone marrow and FB inhibited fibrous tissue proliferation and enhanced hemopoietic and lipid cell viability.
- FB transplantation in rats reduced the duration of leukocytopenia and improved survival following cyclophosphamide treatment.
Conclusions:
- Human fetal bone exhibits a protective effect on bone marrow cells in vitro.
- FB transplantation demonstrates therapeutic potential in mitigating chemotherapy-induced bone marrow suppression and enhancing survival.
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