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A model for evaluating topical antimicrobial efficacy against methicillin-resistant Staphylococcus aureus biofilms in
Eric D Roche1, Paul J Renick, Shannon P Tetens
1Research and Development, Healthpoint Biotherapeutics, Fort Worth, Texas, USA. eric.roche@healthpoint.com
Abstract:
A wound biofilm model was created by adapting a superficial infection model. Partial-thickness murine wounds were inoculated with methicillin-resistant Staphylococcus aureus (MRSA). Dense biofilm communities developed at the wound surface after 24 h as demonstrated by microscopy and quantitative microbiology. Common topical antimicrobial agents had reduced efficacy when treatment was initiated 24 h after inoculation compared to 4 h after inoculation. This model provides a rapid in vivo test for new agents to treat wound biofilm infections.

