Related Experiment Video

Updated: May 21, 2026

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Tumor microRNA-335 expression is associated with poor prognosis in human glioma

Jian Jiang1, Xiaoyang Sun, Weijie Wang

  • 1Department of Neurosurgery, Huaian First Hospital, Nanjing Medical University, Huaian City, 223300, People's Republic of China.

Medical Oncology (Northwood, London, England)
|May 31, 2012
PubMed

Insights

MicroRNA-335 (miR-335) is elevated in human gliomas and linked to advanced tumor progression. High miR-335 expression indicates a poorer prognosis and serves as an independent predictor of clinical outcome in glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-335 (miR-335) has a dual role as a tumor suppressor or promoter.
  • Previous in vitro studies suggested miR-335 acts as an oncogene in glioma cell lines.
  • Its expression and clinical significance in human glioma tissues remained undetermined.

Purpose of the Study:

  • To investigate the clinical significance of miR-335 expression in human glioma tissues.
  • To determine the association between miR-335 expression and clinicopathological factors.
  • To evaluate miR-335 as a prognostic marker for glioma patients.

Main Methods:

  • Real-time quantitative RT-PCR was used to measure miR-335 expression in glioma and nonneoplastic brain tissues.
  • Statistical analysis was performed to correlate miR-335 levels with clinicopathological features and patient survival.
  • Survival analysis, including subgroup analysis by WHO grade, was conducted.

Main Results:

  • miR-335 expression was significantly higher in glioma tissues compared to nonneoplastic brain tissues (P < 0.001).
  • High miR-335 expression correlated with higher WHO tumor grade (P = 0.001) and shorter patient survival (P = 0.01).
  • miR-335 was identified as an independent prognostic factor (P = 0.02), particularly in high-grade gliomas (P = 0.002).

Conclusions:

  • miR-335 expression is upregulated in human gliomas and associated with tumor progression.
  • Elevated miR-335 levels predict a worse clinical outcome for glioma patients.
  • miR-335 serves as a novel and independent biomarker for predicting glioma patient prognosis.

More Related Videos

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...