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Increased NTPDase activity in lymphocytes during experimental sepsis
Claudia de Mello Bertoncheli1, Carine Eloise Prestes Zimmermann, Jeandre Augusto dos Santos Jaques
1Centro de Ciências da Saúde, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
Thescientificworldjournal
|May 31, 2012
Summary
Sepsis in rats increased ectonucleoside triphosphate diphosphohydrolase (NTPDase) activity, specifically breaking down adenosine-5'-triphosphate (ATP). This enzyme activity likely responds to elevated ATP from cell damage during sepsis.
Area of Science:
- Biochemistry
- Immunology
- Pathology
Background:
- Ectonucleoside triphosphate diphosphohydrolase (NTPDase; CD39) modulates immune responses.
- Sepsis is a life-threatening condition characterized by a dysregulated host response to infection.
Purpose of the Study:
- To investigate NTPDase activity in rats with induced sepsis.
- To correlate NTPDase activity with histological changes in vital organs.
Main Methods:
- Sepsis was induced in rats.
- Lymphocytes were isolated from blood 12 hours post-surgery to determine NTPDase activity.
- Histological examination of kidney, liver, and lung tissues was performed.
Main Results:
- A significant increase in adenosine-5 '-triphosphate (ATP) hydrolysis was observed (P < 0.01).
- No significant changes in adenosine-5 '-monophosphate (ADP) hydrolysis were detected (P > 0.05).
- Histological analysis revealed vascular congestion, necrosis, and mononuclear cell infiltration in septic rats.
Conclusions:
- Increased ATP hydrolysis by NTPDase during sepsis may be a compensatory mechanism to clear extracellular ATP released from damaged cells.
- NTPDase activity is altered in sepsis, suggesting a role in the inflammatory and cellular damage processes associated with the condition.
