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Updated: May 21, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Published on: July 21, 2017
Not all autophagy is equal
Maria F Czyzyk-Krzeska1, Jarek Meller, David R Plas
1Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Maria.Czyzykkrzeska@uc.edu
Abstract:
Autophagy is an important mechanism in cancer cell survival and tumor growth and plays both pro- and anti-oncogenic roles. However, the biochemical basis for these diverse functions is not well understood. Our work provides new evidence for the existence of two separate autophagic programs regulated in an opposite manner by the von Hippel-Lindau tumor suppressor (VHL). These programs, marked by differential requirements for LC3B vs. LC3C, play tumor-promoting and tumor-suppressing roles in renal cancer.
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