Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthesis of rhodamine B hydrazine derivatives and their application in glycan analysis.

The Analyst·2026

Same author

Chlorogenic Acid Alleviates Experimental Asthma by Reprogramming DHA Metabolism to Inhibit Ferroptosis.

International journal of molecular sciences·2026

Same author

Ultrasensitive and Specific Detection of 8-Oxoguanine DNA Glycosylase via Locus-Specific Rolling Circle Amplification.

Chemical research in toxicology·2026

Same author

Exploiting Pfu DNA polymerase's differential bypass capacity for AP <i>vs.</i> dU sites: a novel strategy for sensitive uracil-DNA glycosylase detection.

The Analyst·2026

Same author

Research on the migration and transformation patterns of volatile pyrolysis products of WPCB by an online pyrolysis-mass spectrometry method.

RSC advances·2025

Same author

<i>Hyssopus cuspidatus Boriss</i> Volatile Extract (SXC): A Dual-Action Antioxidant and Antifungal Agent Targeting <i>Candida albicans</i> Pathogenicity and Vulvovaginal Candidiasis via Host Oxidative Stress Modulation and Fungal Metabolic Reprogramming.

Antioxidants (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: May 21, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Cocaine detection by structure-switch aptamer-based capillary zone electrophoresis.

Qin-Pei Deng¹, Cai Tie, Ying-Lin Zhou

¹Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry, Peking University, Beijing, China.

Electrophoresis

|June 1, 2012

Summary

A novel aptamer structure-switch strategy enhances small molecule detection using capillary electrophoresis. This method improves sensitivity for detecting compounds like cocaine by monitoring fluorescence changes.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: May 21, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Biochemistry and Molecular Biology
Analytical Chemistry
Biotechnology

Background:

Aptamers are nucleic acid oligonucleotides with high affinity and specificity for targets, widely used as affinity probes in capillary electrophoresis (CE).
Current aptamer-based CE applications face limitations in detecting small molecules due to relatively weak interactions.

Purpose of the Study:

To develop a novel capillary electrophoresis (CE) method for sensitive small molecule detection using the aptamer structure-switch concept.
To investigate the efficacy of this strategy for detecting specific small molecules, such as cocaine.

Main Methods:

A carboxyfluorescein (fluorescein amidite, FAM)-labeled DNA aptamer was incubated with a complementary strand (CS).
Separation and determination of the free aptamer and aptamer-CS duplex were performed using metal cation-mediated CE with laser-induced fluorescence (CE-LIF).
Target introduction destabilized the aptamer-CS duplex, causing changes in fluorescence intensity.

Main Results:

The aptamer structure-switch strategy demonstrated a significant change in fluorescence upon target introduction.
Optimization of the complementary strand (CS) length identified a 12-mer CS as providing the best sensitivity for cocaine detection.
The CE-LIF method effectively combined separation power with specific molecular interactions.

Conclusions:

The developed CE-LIF method, based on the aptamer structure-switch concept, offers a sensitive approach for small molecule detection.
This strategy shows promise as a universal detection method for various aptamer-specified small molecules.
The method overcomes limitations of weak aptamer-small molecule interactions in traditional CE applications.