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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
NF-κB repression by PIAS3 mediated RelA SUMOylation.
Yuangang Liu1, Rebecca Bridges, Aaron Wortham
1Department of Dermatology, Oregon Health and Science University, Portland, Oregon, United States of America. liuy@ohsu.edu
Plos One
|June 1, 2012
Summary
Nuclear factor-kappa B (NF-κB) is negatively regulated by SUMOylation. PIAS3 ligase SUMOylates the RelA subunit of NF-κB, forming a negative feedback loop for homeostasis.
Area of Science:
- Molecular Biology
- Cellular Biology
- Biochemistry
Background:
- Nuclear factor-kappa B (NF-κB) is a crucial transcription factor for tissue homeostasis.
- NF-κB activity is modulated by post-translational modifications like phosphorylation, acetylation, and ubiquitination.
Purpose of the Study:
- To investigate the role of SUMOylation in regulating NF-κB activity.
- To identify the specific proteins involved in NF-κB SUMOylation.
Main Methods:
- Experimental evidence for NF-κB SUMOylation was provided.
- The E3 SUMO ligase activity of PIAS3 in regulating NF-κB was examined.
- Mutant analysis of RelA and PIAS3 was performed.
Main Results:
- The RelA subunit of NF-κB was found to be SUMOylated by PIAS3.
- PIAS3-mediated repression of NF-κB was dependent on SUMOylation.
- NF-κB activation induced PIAS3-mediated SUMOylation of RelA, creating a negative feedback loop.
- RelA DNA binding was identified as a signal for PIAS3-mediated SUMOylation.
Conclusions:
- NF-κB is regulated by SUMOylation via the PIAS3 ligase.
- PIAS3-mediated RelA SUMOylation represents a novel negative feedback mechanism for NF-κB regulation.
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