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Related Experiment Video

Updated: May 21, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
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Published on: February 24, 2018

NAD (+) -dependent Formate Dehydrogenase from Plants.

A A Alekseeva1, S S Savin, V I Tishkov

  • 1Chemistry Department, Lomonosov Moscow State University.

Acta Naturae
|June 1, 2012
PubMed
Summary

Plant formate dehydrogenases (FDHs) are crucial mitochondrial enzymes. This review highlights recent advances in understanding their roles, characteristics, structure, and potential for protein engineering, contrasting them with microbial counterparts.

Keywords:
physiological roleplant formate dehydrogenasepropertiesprotein engineeringstructure; expression;Escherichia coli

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Area of Science:

  • Biochemistry
  • Plant Biology
  • Enzymology

Background:

  • NAD(+)-dependent formate dehydrogenase (FDH) is a widespread enzyme found in various organisms, including plants, bacteria, yeasts, and fungi.
  • Unlike microbial FDHs in the cytoplasm, plant FDHs are localized in mitochondria.
  • Plant FDHs have been less studied compared to microbial FDHs, despite their discovery in 1921.

Purpose of the Study:

  • To review recent findings on plant formate dehydrogenases.
  • To summarize their physiological roles, properties, and structure.
  • To discuss recent developments in the protein engineering of plant FDHs.

Main Methods:

  • Literature review of recent studies on plant FDHs.
  • Analysis of existing data on enzyme properties, localization, and structure.
  • Examination of research on protein engineering applications.

Main Results:

  • Plant FDHs are mitochondrial enzymes with a homodimeric structure, lacking prosthetic groups or metal ions.
  • Recent research has elucidated their physiological significance and biochemical properties.
  • Progress has been made in understanding and engineering plant FDHs.

Conclusions:

  • Plant FDHs are significant mitochondrial enzymes with unique characteristics.
  • Continued research is vital for fully understanding their biological roles and harnessing their potential through protein engineering.