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Updated: May 21, 2026
Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells
Published on: July 10, 2019
[Regulation of BDNF expression in traumatic brain injury rats subjected single neucleated cell transplantation]
Nan Zhao1, Qi-Qin Dan, Jun Liu
1Department of Neurosurgery, No. 1 Renming Hospital, Affiliated Kunming City, Kunming 650031, China.
Objective:
To investigate the effect of single neucleated cells transplantation on the neurological function and brain derived neurotrophic factor (BDNF) expression in traumatic brain injury (TBI) rats.
Methods:
TBI rats were established by hammer fall method from 30 cm height with 50 g weight. Single neucleated cells/ karyocytes from bone marrow were separated and prepared by ficoll medium, then transplanted into pericontusional tissues in the brain. Neurological function severity scores (NSS) were recorded at 1, 3, and 7 days post operation (dpo). The pericontusional tissues were harvested at 7 dpo to analyze the BDNF localization, expressional level.
Results:
Single neucleated cell transplantation decreased NSS significantly, compared with TBI rats without cell implantation (P < 0.05). BDNF expression was upregulated and mainly found in neurons.
Conclusion:
The present study showed the single karyocytes transplantation could improve neurological function and the mechanism is possibly linked to the BDNF expression.
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