Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Euphol targets key oncogenic processes and promotes chemoprevention in organoid and In Vivo models of colorectal cancers.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

High-dose chemotherapy with autologous stem cell rescue in children under 5 years of age with central nervous system embryonal tumors: results from a prospective cohort in an upper-middle-income country.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2026
Same author

Anti-miR-195-engineered mesenchymal stem cells promote migration, vascularization, and ECM protein expression in vivo.

Stem cell research & therapy·2026
Same author

Tumor-Associated Fusobacterium nucleatum and Aggressive Architectural Features in Stage II Colorectal Cancer.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same author

On the potential origin of the zygote-like cancer stem cell with a focus on fusion for cell rescue.

Frontiers in cell and developmental biology·2026
Same author

Evaluating eight smoking metrics for modelling survival in non-small cell lung cancer.

Cancer epidemiology·2026

Related Experiment Video

Updated: May 21, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Decoy activity through microRNAs: the therapeutic implications.

Maria Ines Almeida1, Rui Manuel Reis, George Adrian Calin

  • 1The University of Texas MD Anderson Cancer Center, Department of Experimental Therapeutics, Houston, TX 77030, USA.

Expert Opinion on Biological Therapy
|June 2, 2012
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) act as decoys, interacting with other RNAs or proteins to regulate gene expression. This biological decoy activity has significant implications for developing new therapies for diseases like cancer.

More Related Videos

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Related Experiment Videos

Last Updated: May 21, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Noncoding RNAs

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression post-transcriptionally.
  • Dysregulation of miRNAs is implicated in various diseases, including cancer.
  • miRNAs control mRNA translation or degradation by binding to target messenger RNAs (mRNAs).

Purpose of the Study:

  • To review studies investigating the role of miRNAs in biological decoy mechanisms.
  • To explore the RNA-RNA language involving competing endogenous RNAs (ceRNAs).
  • To understand how miRNAs interact with proteins that bind mRNAs.

Main Methods:

  • Literature review of seminal contributions on miRNA decoy functions.
  • Analysis of studies describing miRNA cross-talk through decoy mechanisms.
  • Examination of miRNA interactions with competing endogenous RNAs and protein complexes.

Main Results:

  • miRNAs function as components of a biological decoy system.
  • Competing endogenous RNAs can sequester miRNAs, influencing gene expression.
  • miRNAs can also be trapped by proteins with mRNA binding sites, acting as decoys.

Conclusions:

  • miRNA decoy functions offer novel therapeutic strategies for human diseases.
  • Understanding these mechanisms can help overcome drug resistance.
  • This knowledge is crucial for designing future miRNA-based clinical trials.