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Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
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Cell-derived matrix coatings for polymeric scaffolds.

Martin L Decaris1, Bernard Y Binder, Matthew A Soicher

  • 1Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA.

Tissue Engineering. Part A
|June 2, 2012
PubMed
Summary
This summary is machine-generated.

Decellularized extracellular matrices (DMs) from human mesenchymal stem cells (MSCs) were successfully transferred to 3D scaffolds. These transferred DMs (tDMs) enhanced osteogenic differentiation and vascularization in vitro and in vivo.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Cells deposit extracellular matrices (DMs) that influence cell behavior.
  • Applying DMs to 3D scaffolds is challenging due to deposition and transport issues.

Purpose of the Study:

  • To investigate transferring 2D-cultured DMs from human mesenchymal stem cells (MSCs) to 3D polymeric scaffolds.
  • To determine if transferred DMs (tDMs) retain their ability to direct cell fate and enhance osteogenic differentiation.

Main Methods:

  • Human MSCs cultured in 2D to form DMs.
  • DMs were transferred to polymeric scaffolds (tDM-coated scaffolds).
  • Osteogenic differentiation and subcutaneous implantation in nude rats were assessed.

Main Results:

  • tDM-coated scaffolds enhanced osteogenic marker gene expression (BGLAP, IBSP) and alkaline phosphatase in MSCs.
  • Undifferentiated MSCs on tDM-coated scaffolds showed increased calcium deposition.
  • Implanted tDM-coated scaffolds promoted higher blood vessel density after 2 weeks.

Conclusions:

  • 2D-derived DM coatings can be successfully transferred to 3D substrates.
  • Transferred DMs maintain their capacity to modulate cell phenotype, promoting osteogenesis and vascularization.