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Published on: December 23, 2022
A novel UPLC-MS/MS method for simultaneous quantification of rhein, emodin, berberine and baicalin in rat plasma and
Ping Huang1, Jing-wen Gao, Zhan Shi
1Laboratory of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
Background:
Dahuang Huanglian Xiexin Decoction (DHXD) is a classical formula in traditional Chinese medicines. In this study, a novel UPLC-MS/MS method was developed for the simultaneous quantification of rhein, emodin, berberine and baicalin, the major bioactive compounds of DHXD in rat plasma.
Results:
The method possessed high sensitivity and ultrashort analysis time (7 min). Linearity, accuracy, precision and extraction recovery of four analytes were all satisfactory. The method was then successfully applied in a pharmacokinetic study of four bioactive components after a single oral administration of DHXD extract to rats.
Conclusion:
The method was applicable for simultaneous bioanalysis of rhein, emodin, berberine and baicalin.
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