Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[An integrated electrical stimulation-drug iontophoresis-monitoring system for tendon injury repair].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2026

Same author

DH-OOD: A decoupled hybrid framework for robust skin lesion classification via semantic-structural fusion.

Journal of X-ray science and technology·2026

Same author

Loganin alleviates oxidative stress-induced apoptosis by modulating mitochondrial function and STAT3 signaling in DSS-induced colitis and H<sub>2</sub>O<sub>2</sub>-injured Caco-2 cells.

Scientific reports·2026

Same author

The <i>Ascosphaera apis</i> Invasion of <i>Apis cerana</i> Worker Larvae: Long Non-Coding RNA-Mediated Regulation.

Biology·2026

Same author

Revealing the Modulatory Role of Microsporidian circRNAs in the Infection of Honey Bee Workers.

Insects·2026

Same author

Ascosphaeraapis LncRNA6140 promotes the infection of honeybee larvae through the milR5658-x-ATPase axis.

Insect biochemistry and molecular biology·2026

Search research articles

Related Experiment Video

Updated: May 21, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

An ascorbic acid amperometric sensor using over-oxidized polypyrrole and palladium nanoparticles composites.

Wentao Shi¹, Chunxiu Liu, Yilin Song

¹State Key Laboratory of Transducer, Institute of Electronics, Chinese Academy of Science, Beijing 100190, PR China.

Biosensors & Bioelectronics

|June 2, 2012

Summary

We developed a sensitive electrochemical sensor using over-oxidized polypyrrole and palladium nanoparticles for detecting ascorbic acid (AA). This sensor offers rapid detection with high sensitivity and a low limit of detection, proving useful in complex biological systems.

More Related Videos

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Related Experiment Videos

Last Updated: May 21, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Area of Science:

Electrochemistry
Nanomaterials Science
Biosensors

Background:

Ascorbic acid (AA) is a vital biomolecule with numerous physiological roles.
Developing sensitive and selective electrochemical sensors for AA is crucial for diagnostics and research.
Existing methods may face limitations in sensitivity, response time, or applicability in complex matrices.

Purpose of the Study:

To construct a highly responsive electrochemical sensor for ascorbic acid (AA) detection.
To utilize a composite of over-oxidized polypyrrole (OPPy) and palladium nanoparticles (PdNPs) for enhanced sensor performance.
To evaluate the sensor's sensitivity, response time, limit of detection, and stability.

Main Methods:

Fabrication of an over-oxidized polypyrrole (OPPy) and palladium nanoparticles (PdNPs) composite on a gold electrode (OPPy-PdNPs/Au).
Characterization of PdNPs using UV-vis spectroscopy and TEM; surface analysis of OPPy-PdNPs/Au using FE-SEM.
Electrochemical measurements including cyclic voltammetry (CV) to assess AA oxidation catalysis and current response across different concentrations.

Main Results:

The OPPy-PdNPs/Au electrode effectively catalyzed AA oxidation, lowering its oxidation potential to 0 V.
The sensor demonstrated two linear concentration ranges for AA detection (1–520 μM and 120–1600 μM) with high sensitivity (570 μA mM⁻¹cm⁻²) in the lower range.
Achieved a short response time (<2s) and a very low limit of detection (1 μM), with good reproducibility and interference resistance.

Conclusions:

The developed OPPy-PdNPs/Au electrochemical sensor is simple, cost-effective, and highly sensitive for AA detection.
The sensor exhibits excellent performance characteristics, including rapid response and low detection limits.
This sensor holds significant potential for detecting AA in complex biological samples, particularly in neuroscience applications.