Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cytoskeletal Coordination in Cell Migration

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...

Assembly of Signaling Complexes

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

Introduction to the Cytoskeleton

Introduction to the Cytoskeleton

Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶ microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their homologs were...

The Role of Actin and Myosin in Non-muscle Cells

The Role of Actin and Myosin in Non-muscle Cells

Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They are held...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Colchicine-BODIPY Probes: Evidence for the Involvement of Intracellular Membranes in the Targeting of Colchicine to Tubulin.

ACS pharmacology & translational science·2025

Same author

Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C.

Cancer cell international·2025

Same author

Discovery of a 6-Aminobenzo[<i>b</i>]thiophene 1,1-Dioxide Derivative (K2071) with a Signal Transducer and Activator of Transcription 3 Inhibitory, Antimitotic, and Senotherapeutic Activities.

ACS pharmacology & translational science·2024

Same author

Click estradiol dimers with novel aromatic bridging units: synthesis and anticancer evaluation.

Journal of enzyme inhibition and medicinal chemistry·2024

Same author

Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.

Frontiers in immunology·2024

Same author

γ-Tubulin in microtubule nucleation and beyond.

Frontiers in cell and developmental biology·2022

Search research articles

Related Experiment Video

Updated: May 21, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Cytoskeleton in mast cell signaling.

Pavel Dráber¹, Vadym Sulimenko, Eduarda Dráberová

¹Department of Biology of Cytoskeleton, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic Prague, Czech Republic.

Frontiers in Immunology

|June 2, 2012

Summary

Mast cell activation involves cytoskeletal reorganization, crucial for releasing inflammatory mediators. This review details how microtubules, microfilaments, and intermediate filaments regulate mast cell signaling and function.

Keywords:

actins intermediate filaments mast cell activation microfilaments microtubules signal transduction tubulins vimentin

More Related Videos

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Published on: July 4, 2018

Related Experiment Videos

Last Updated: May 21, 2026

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

Published on: July 4, 2018

Area of Science:

Immunology
Cell Biology
Biochemistry

Background:

Mast cell activation via FcεRI is central to allergic responses and inflammation.
Co-receptors like c-Kit and GPCRs amplify FcεRI-mediated inflammatory mediator release.
Cytoskeletal dynamics are essential for mast cell signaling, morphology, adhesion, exocytosis, and migration.

Purpose of the Study:

To critically review the role of the cytoskeleton in mast cell signaling.
To elucidate the involvement of microtubules, microfilaments, and intermediate filaments in mast cell activation.

Main Methods:

Literature review and critical appraisal of existing research.
Analysis of signaling pathways affecting cytoskeletal proteins (kinases, phosphatases, Rho GTPases, Ca2+).
Examination of nucleation proteins (γ-tubulin, Arp 2/3 complex) and associated factors.

Main Results:

Cytoskeletal reorganization, particularly microtubules and microfilaments, is pivotal during mast cell activation.
Actin polymerization, tubulin dynamics, and intermediate filament (vimentin) changes are modulated by signaling pathways.
Nucleation proteins and regulatory factors govern the dynamic nature of cytoskeletal components.

Conclusions:

The cytoskeleton plays a fundamental role in mast cell activation and inflammatory mediator release.
Understanding cytoskeletal regulation provides insights into allergic responses and potential therapeutic targets.