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Electrophoresis: Overview

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
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Related Experiment Video

Updated: May 21, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform.

Mark C Draper¹, Xize Niu, Soongwon Cho

¹Department of Chemistry, Imperial College London, Kensington, London, United Kingdom.

Analytical Chemistry

|June 5, 2012

Summary

This study integrates microfluidics with microcapillary gel electrophoresis to isolate and store separated analytes in microdroplets. The system enables precise control for applications like concentration gradient generation.

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Last Updated: May 21, 2026

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Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Area of Science:

Analytical Chemistry
Biochemistry
Microfluidics

Background:

Microfluidic systems offer precise control over sample manipulation.
Microcapillary gel electrophoresis (μCGE) is effective for separating analytes.
Integrating these technologies can enhance sample handling and analysis.

Purpose of the Study:

To develop a monolithic system integrating multiphase microfluidics with μCGE.
To achieve complete isolation and storage of separated analyte bands.
To explore the system's potential as a concentration gradient generator.

Main Methods:

Monolithic integration of a multiphase microfluidic system and μCGE architecture.
Separation of fluorescent analytes using microchannel gel electrophoresis.
Encapsulation of eluted analyte bands into 40-600 microdroplets with controlled size, shape, and composition.

Main Results:

Successful separation, optical detection, and encapsulation of two fluorescent analytes.
Demonstrated precise control over microdroplet characteristics.
Investigated the system's capability for generating concentration gradients from segmented analyte bands.

Conclusions:

The integrated μCGE and microfluidic system provides a robust platform for analyte isolation and storage.
The system offers precise control over microdroplet composition for downstream applications.
This technology shows promise for advanced applications such as concentration gradient generation.