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Updated: May 21, 2026

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
Published on: February 23, 2020
Multianalyte digital enzyme biosensors with built-in Boolean logic
Evgeny Katz1, Joseph Wang, Marina Privman
1Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, New York 13699, United States. ekatz@clarkson.edu
Abstract:
Novel biosensors based on the biocomputing concept digitally process multiple biochemical signals through Boolean logic networks of coupled biomolecular reactions and produce output in the form of a YES/NO response. Compared to traditional single-analyte sensing devices, biocomputing approach enables a high-fidelity multianalyte biosensing, particularly beneficial for biomedical applications.
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Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

