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Related Experiment Videos

Decrease in hepatic mitochondrial redox state by massive infusion of citrate-phosphate-dextrose solution in jaundiced

Y Wada1, K Mori, H Egawa

  • 1Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.

European Surgical Research. Europaische Chirurgische Forschung. Recherches Chirurgicales Europeennes
|January 1, 1990
PubMed
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Massive blood transfusions can impair liver function, particularly in damaged livers. Studies show a decrease in arterial ketone body ratio, indicating metabolic derangement in jaundiced rabbits receiving citrate-phosphate-dextrose solution.

Area of Science:

  • Hepatology
  • Metabolic biochemistry
  • Transfusion medicine

Background:

  • Massive blood transfusions are critical in trauma care but can lead to metabolic complications.
  • The liver's role in metabolism makes it vulnerable to derangements during transfusion, especially when damaged.
  • Understanding the hepatic mitochondrial redox state is key to elucidating these metabolic changes.

Purpose of the Study:

  • To investigate the mechanism of metabolic derangement caused by massive blood transfusion in a damaged liver model.
  • To assess changes in the hepatic mitochondrial redox state using the arterial ketone body ratio.
  • To evaluate the impact of citrate-phosphate-dextrose (CPD) solution infusion on metabolic parameters.

Main Methods:

  • Jaundiced rabbits with common bile duct ligation (BDL group) and a sham group underwent a 3-hour infusion of CPD solution (9 ml/kg/h).

Related Experiment Videos

  • Arterial blood was analyzed for blood gas, pyruvate, lactate, citrate, and ketone body ratio (acetoacetate/3-hydroxybutyrate).
  • Hepatic mitochondrial redox state was inferred from the arterial ketone body ratio.

    • Main Results:

    • The arterial ketone body ratio significantly decreased in the BDL group, correlating with severe metabolic acidosis (p < 0.01).
    • In contrast, the sham group maintained a high arterial ketone body ratio with metabolic alkalosis.
    • Higher accumulation of organic acids was observed in the BDL group compared to the sham group.

    Conclusions:

    • Massive blood transfusion, particularly via CPD solution, induces metabolic derangement in damaged livers.
    • The observed decrease in arterial ketone body ratio indicates impaired hepatic mitochondrial function.
    • These findings suggest a significant hepatodepressant effect of massive blood transfusion in compromised liver conditions.