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Several distinctive characteristics distinguish glutathione conjugation from other phase II...
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Related Experiment Video

Updated: May 21, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

Glutathione efflux and cell death.

Rodrigo Franco1, John A Cidlowski

  • 1Redox Biology Center, University of Nebraska-Lincoln, Lincoln, NE 68583, USA. rfrancocruz2@unl.edu

Antioxidants & Redox Signaling
|June 5, 2012
PubMed
Summary

Glutathione (GSH) depletion, through efflux, actively regulates cell death signaling. This process impacts cellular redox balance independently of oxidative stress, highlighting GSH transport

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Signaling Pathways

Background:

  • Glutathione (GSH) loss is increasingly recognized as an active regulator of cell death.
  • GSH's role extends beyond antioxidant defense to active signaling in cell death.
  • This active phenomenon modulates redox signaling events crucial for cell death activation.

Purpose of the Study:

  • To review the role of glutathione (GSH) depletion via efflux in cell death.
  • To explore GSH efflux as a regulator of cellular redox balance independent of oxidative stress.
  • To discuss mechanisms of GSH efflux and its signaling in cell death activation.

Main Methods:

  • Literature review focusing on GSH depletion and cell death.
  • Analysis of mechanisms driving GSH efflux.

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Published on: October 10, 2020

  • Examination of redox signaling pathways regulated by GSH depletion.

    • Main Results:

    • GSH depletion by efflux is a key event in cell death.
    • This process alters cellular redox balance independently of reactive oxygen and nitrogen species (ROS/RNS).
    • GSH efflux mechanisms and their role in activating cell death machinery are discussed.

    Conclusions:

    • GSH transport is central to redox signaling during cell death.
    • Identifying molecular GSH transporters involved in cell death is a future research direction.
    • Developing sensitive methods to quantify GSH efflux is needed.