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Related Experiment Videos

Gap junctional permeability is affected by cell volume changes and modulates volume regulation.

A Ngezahayo1, H A Kolb

  • 1University of Konstanz, Faculty of Biology, FRG.

FEBS Letters
|December 10, 1990
PubMed
Summary

Mild hypotonic shock uncoupled pancreatic acinar cells, revealing a link between cell volume and gap junction channels. A G-protein dependent pathway, modulated by protein kinase C (PKC), regulates this process.

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Area of Science:

  • Cell biology
  • Physiology
  • Biochemistry

Background:

  • Gap junction channels mediate communication between adjacent cells.
  • Pancreatic acinar cells play a crucial role in digestion.
  • Cell volume regulation is essential for cellular function.

Purpose of the Study:

  • To investigate the relationship between cell volume changes and gap junctional communication in pancreatic acinar cells.
  • To identify the signaling pathways involved in hypotonicity-induced electrical uncoupling.

Main Methods:

  • Using isolated pancreatic acinar cell pairs.
  • Employing the double whole-cell patch-clamp technique.
  • Utilizing GTP[S] dialysis, cytochalasin B treatment, and protein kinase C (PKC) inhibition with polymyxin B.

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Main Results:

  • Hypotonic shock caused electrical uncoupling of pancreatic acinar cell pairs.
  • This uncoupling was delayed but could be accelerated by GTP[S] and cytochalasin B.
  • Protein kinase C (PKC) inhibition blocked the uncoupling effect.
  • Cell volume changes were correlated with gap junctional permeability.

Conclusions:

  • A G-protein dependent mechanism regulates gap junctional communication in response to cell swelling.
  • Protein kinase C (PKC) is involved in the hypotonicity-induced electrical uncoupling.
  • Gap junctional coupling appears to modulate cellular volume regulation.