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Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens
L B Tang1, R Lenstra, T V Borchert
1Central Research and Development Division, E.I. duPont de Nemours Company, Wilmington, DE 19880-0228.
Abstract:
The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis. Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region. The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
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