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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

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Related Experiment Video

Updated: May 21, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Computational methods for electron tomography.

Jose-Jesus Fernandez1

  • 1National Centre for Biotechnology, National Research Council (CNB-CSIC), Campus UAM, C/Darwin 3, Cantoblanco, 28049 Madrid, Spain. jj.fernandez@csic.es

Micron (Oxford, England : 1993)
|June 5, 2012
PubMed
Summary
This summary is machine-generated.

Electron tomography (ET) visualizes molecular structures in cells and viruses at nanometer resolution. This review covers computational methods and high-performance computing (HPC) for advanced biological imaging.

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Last Updated: May 21, 2026

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Area of Science:

  • Molecular and Cellular Biology
  • Structural Biology
  • Biophysics

Background:

  • Electron tomography (ET) provides high-resolution 3D visualization of biological samples.
  • Recent advancements in ET have led to significant breakthroughs in understanding biological processes.
  • ET is crucial for studying the molecular architecture of viruses, organelles, and cells.

Purpose of the Study:

  • To comprehensively review computational methods and technologies for structural studies using ET.
  • To describe the computational stages from image acquisition to tomogram interpretation.
  • To briefly explain high-performance computing (HPC) techniques used in ET.

Main Methods:

  • Sample preparation for electron microscopy.
  • Image acquisition at multiple views.
  • Computational processing for 3D reconstruction (tomogram generation).
  • Post-reconstruction computational steps: noise reduction, segmentation, and subvolume analysis.

Main Results:

  • ET enables visualization of molecular architecture at nanometer resolution.
  • Computational processing and HPC are essential for managing the data-intensive nature of ET.
  • ET has provided key insights into diverse biological mechanisms.

Conclusions:

  • ET is a powerful tool for molecular and cellular biology research.
  • Effective computational strategies and HPC are critical for successful ET-based structural studies.
  • This review consolidates knowledge on ET computational workflows and HPC applications.