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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...

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Related Experiment Video

Updated: May 21, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Expression pattern of SVEP1 alternatively-spliced forms.

Chen Glait-Santar1, Metsada Pasmanik-Chor, Dafna Benayahu

  • 1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Israel.

Gene
|June 5, 2012
PubMed
Summary
This summary is machine-generated.

This study reveals how the SVEP1 gene

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Published on: October 9, 2014

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Biology

Background:

  • The SVEP1 gene encodes a cell adhesion molecule (CAM) involved in cellular interactions.
  • Understanding SVEP1 regulation is crucial, especially in contexts like bone metastasis where cellular crosstalk occurs.

Purpose of the Study:

  • To investigate the regulation of an alternative promoter (AP) in the SVEP1 gene.
  • To analyze the expression of SVEP1 alternative splice forms in response to cellular interactions and signaling molecules.

Main Methods:

  • Differential expression analysis of SVEP1 isoforms in pre-osteoblastic and mammary adenocarcinoma cells.
  • Chromatin immunoprecipitation (ChIP) assay to identify transcription factor binding to the SVEP1 AP.
  • Treatment with epigenetic modifiers (5'-aza-deoxycitidine, Trichostatin-A) to assess methylation and expression.

Main Results:

  • SVEP1 isoforms are differentially expressed between MBA-15 and DA3 cell lines.
  • Co-culturing and treatment with TNFα or estrogen alter SVEP1 isoform levels, with isoforms a and e increasing upon co-culture.
  • Transcription factor binding to the SVEP1 AP varies between cell types, and epigenetic modifications influence gene expression.

Conclusions:

  • SVEP1 alternative splicing and promoter activity are regulated by cellular crosstalk and specific signaling pathways.
  • These regulatory mechanisms may be significant in the bone microenvironment during carcinoma cell invasion.
  • The findings offer insights into SVEP1's role in the context of bone metastasis.