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Phosphoprotein abundance changes in hypertensive cardiac remodeling
Kumar Kotlo1, Keven R Johnson, Jean M Grillon
1Department of Medicine, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA.
Journal of Proteomics
|June 5, 2012
Summary
This study identifies key phosphoproteins involved in cardiac hypertrophy and heart failure using global phosphoprotein profiling. These findings advance our understanding of the molecular mechanisms driving heart disease progression.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Molecular Cardiology
Background:
- Protein phosphorylation is crucial for regulating cardiac function and remodeling.
- Various kinases and phosphatases control cardiomyocyte growth and contractility.
Purpose of the Study:
- To identify phosphorylated proteins associated with pressure overload (PO) cardiac hypertrophy and heart failure.
- To analyze phosphoprotein profiles in established rat models of cardiac dysfunction.
Main Methods:
- Global phosphoprotein profiling using 2-dimensional difference in gel electrophoresis (2D-DIGE).
- Phospho-enriched proteins analyzed from hypertrophic and failing hearts.
- Identification of phosphoproteins by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and Mascot analysis.
Main Results:
- Identified 25 common phosphoproteins with altered abundance in cardiac hypertrophy and/or systolic failure models.
- Key identified proteins include myofilament, mitochondrial, and phosphatase proteins.
- Novel phosphoproteins in cardiac hypertrophy and failure were discovered.
Conclusions:
- Global phosphoprotein profiling effectively identifies proteins involved in cardiac hypertrophy and heart failure.
- The study provides a comprehensive list of phosphoproteins implicated in cardiac remodeling and dysfunction.
- Findings contribute to understanding the molecular basis of heart disease and may reveal therapeutic targets.
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