Multiview light-sheet microscope for rapid in toto imaging
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Updated: May 21, 2026

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
Published on: May 5, 2014
Uros Krzic1, Stefan Gunther, Timothy E Saunders
1Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Abstract:
We present a multiview selective-plane illumination microscope (MuVi-SPIM), comprising two detection and illumination objective lenses, that allows rapid in toto fluorescence imaging of biological specimens with subcellular resolution. The fixed geometrical arrangement of the imaging branches enables multiview data fusion in real time. The high speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we demonstrate through in toto imaging of the embryonic development of Drosophila melanogaster.
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