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Related Experiment Video

Updated: May 21, 2026

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Published on: May 5, 2014

Multiview light-sheet microscope for rapid in toto imaging.

Uros Krzic¹, Stefan Gunther, Timothy E Saunders

¹Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

|June 5, 2012

Summary

This summary is machine-generated.

We developed a new microscope for fast, high-resolution 3D imaging of whole organisms. This advanced microscopy technique allows real-time tracking of cellular dynamics during embryonic development.

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Last Updated: May 21, 2026

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

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Published on: May 5, 2014

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Area of Science:

Biophysics
Developmental Biology
Microscopy

Background:

High-resolution imaging of whole biological specimens is crucial for understanding complex developmental processes.
Existing microscopy techniques often face limitations in speed, resolution, or sample invasiveness.

Purpose of the Study:

To introduce and validate a novel multiview selective-plane illumination microscope (MuVi-SPIM).
To demonstrate the capability of MuVi-SPIM for rapid, in toto, subcellular resolution fluorescence imaging.

Main Methods:

Utilized a multiview selective-plane illumination microscope (MuVi-SPIM) setup with dual detection and illumination objectives.
Implemented real-time multiview data fusion enabled by a fixed geometrical arrangement of imaging branches.
Performed in toto imaging of embryonic development in Drosophila melanogaster.

Main Results:

Achieved rapid in toto fluorescence imaging of biological specimens with subcellular resolution.
Demonstrated faithful tracking of nuclei and cell shape changes during embryonic development.
Successfully captured dynamic cellular processes in real time.

Conclusions:

MuVi-SPIM enables high-speed, high-resolution 3D imaging of whole organisms.
The system facilitates real-time observation of cellular dynamics during development.
This technique offers a powerful tool for developmental biology research.