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Related Experiment Video

Updated: May 21, 2026

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
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Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

Gravity-oriented microfluidic device for uniform and massive cell spheroid formation.

Kangsun Lee, Choong Kim, Jae Young Yang

    Biomicrofluidics
    |June 5, 2012
    PubMed
    Summary
    This summary is machine-generated.

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    We developed a simple microfluidic method using gravity to create uniform, large 3-D cell spheroids. This technique allows for easy formation, perfusion, and retrieval of cell models for research.

    Area of Science:

    • Biotechnology
    • Microfluidics
    • Cell Biology

    Background:

    • Three-dimensional (3-D) cell culture models are crucial for studying cellular processes like proliferation, differentiation, and metabolism.
    • Existing methods for spheroid formation can be complex and lack scalability.

    Purpose of the Study:

    • To present a novel, user-friendly microfluidic method for generating massive and uniform 3-D cell spheroids.
    • To demonstrate the feasibility of spheroid formation, perfusion, and retrieval using gravitational force in a vertically oriented device.

    Main Methods:

    • Utilized a multi-level structured microfluidic device oriented vertically.
    • Employed gravitational force to facilitate the formation of cell spheroids.
    • Incorporated standard pipetting for spheroid retrieval and long-term perfusion.

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    Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
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    Generation of Three-Dimensional Spheroids/Organoids from Two-Dimensional Cell Cultures Using a Novel Stamp Device
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    Generation of Three-Dimensional Spheroids/Organoids from Two-Dimensional Cell Cultures Using a Novel Stamp Device

    Published on: March 28, 2025

    Related Experiment Videos

    Last Updated: May 21, 2026

    Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids
    10:27

    Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

    Published on: November 7, 2019

    Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
    06:39

    Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

    Published on: October 6, 2022

    Generation of Three-Dimensional Spheroids/Organoids from Two-Dimensional Cell Cultures Using a Novel Stamp Device
    05:40

    Generation of Three-Dimensional Spheroids/Organoids from Two-Dimensional Cell Cultures Using a Novel Stamp Device

    Published on: March 28, 2025

    Main Results:

    • Successfully formed uniform, size-controllable, and well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293).
    • Demonstrated the capability for long-term perfusion and easy retrieval of the cultured spheroids.
    • The gravity-driven method ensures consistent spheroid generation.

    Conclusions:

    • The proposed gravity-assisted microfluidic technique offers a simple and effective approach for 3-D spheroid culture.
    • This method is a valuable tool for in-vitro studies of embryoid bodies and tumor models.
    • Facilitates research on cell proliferation, differentiation, and metabolism in 3-D environments.