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Histamine blocks interleukin 2 (IL-2) gene expression and regulates IL-2 receptor expression
A R Rezai1, J F Salazar-Gonzalez, O Martínez-Maza
1Department of Microbiology & Immunology, UCLA School of Medicine 90024.
Immunopharmacology and Immunotoxicology
|January 1, 1990
Summary
Histamine inhibits T cell proliferation by blocking interleukin-2 (IL-2) gene expression via H2 receptors. It also modulates IL-2 receptor expression, particularly in AIDS-KS patients.
Area of Science:
- Immunology
- Cell Biology
- Pharmacology
Background:
- Histamine is a key mediator in immune responses.
- T cell proliferation is crucial for adaptive immunity.
- Interleukin-2 (IL-2) is vital for T cell growth and function.
Purpose of the Study:
- To investigate the effect of histamine on T cell proliferation.
- To elucidate the mechanism underlying histamine's influence on IL-2 production and receptor expression.
- To explore histamine's immunomodulatory role in AIDS-KS patients.
Main Methods:
- Human peripheral blood mononuclear cells (PBMC) were stimulated with Phytohemagglutinin-P (PHA-P).
- Histamine's effect on proliferation was assessed with and without H1/H2 antagonists (Diphenhydramine, Cimetidine).
- IL-2 gene expression, secreted IL-2, and IL-2 receptor expression were measured via RT-PCR and flow cytometry.
Main Results:
- Histamine inhibited PHA-P-induced PBMC proliferation in a dose-dependent manner, mediated by H2 receptors.
- Histamine suppressed IL-2 gene expression and secretion, but not IL-2 receptor expression.
- Histamine treatment reversed the anti-proliferative effect and enhanced IL-2 receptor expression in some donors, notably in AIDS-KS patients.
Conclusions:
- Histamine inhibits T cell proliferation by blocking IL-2 gene expression and production.
- Histamine exhibits immunomodulatory effects on IL-2 receptor expression, particularly in individuals with AIDS-KS.
- These findings highlight histamine's complex role in regulating T cell immunity.