Related Experiment Video
Updated: May 21, 2026

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
Published on: June 17, 2020
Urine is a highly cytotoxic agent: does it influence stem cell therapies in urology?
J Adamowicz1, T Kloskowski, J Tworkiewicz
1Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, Poland. adamowicz.jz@gmail.com
Abstract:
The state of art of stem cell therapies in urologic regenerative medicine is still under development. There are still many issues before advances in tissue engineering can be introduced for clinical application. The essential question is whether stem cells should be seeded on the urinary tract lumen side. The present experiment, using Real-Time Cell Analyzer (RTCA) DP (Dual Plate) of the xCellligence system (Roche Applied Science, Mannheim, Germany), allowed us to monitor cellular events in real time. In this study we examined the influence of urine on bone marrow-derived mesenchymal stem cells (MSC). Cells were exposed to medium mixed with urine (1:1), medium mix with PBS (Phosphate Buffered Saline) (1:1), only urine, and whole medium without cells as background. The cell number was significantly lower in all groups exposed on medium mixed with urine and urine alone. The results showed that urine is a highly cytotoxic agent whose role in urologic regenerative medicine is underestimated.
Related Concept Videos
Stem Cell Therapy for Tissue Regeneration
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
Stem Cell Culture
Kidney Transplant II: Surgical Procedure
Physiology of Urine Formation
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
Regulation of Hematopoietic Stem Cells
Urine Studies II: Urine Culture and Sensitivity Test

