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Presumptive identification of Mycobacterium tuberculosis complex based on cord formation in BACTEC MGIT 960 medium
1Department of Microbiology, Lala Ram Swarup Institute of Tuberculosis and Respiratory Diseases, New Delhi 110 030, India.
Abstract:
We considered samples received for culture of mycobacteria using BACTEC MGIT 960 system over a period of 1 year. Tubes flagged positive by MGIT were evaluated for presence of serpentine cording. The cord formation was compared with isolates identified as Mycobacterium tuberculosis complex (MTC) based on p-nitrobenzoic acid (PNB) test. Cords were found in 591 isolates of which 584 (98.8%) were confirmed as MTC. The sensitivity and specificity of cord formation were found to be 99.7% and 89.9%, respectively.
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