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Updated: May 21, 2026

Collection Protocol for Human Pancreas

Collection Protocol for Human Pancreas

Published on: May 23, 2012

Collection protocol for human pancreas.

Martha L Campbell-Thompson¹, Emily L Montgomery, Robin M Foss

¹Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, USA. mct@ufl.edu

Journal of Visualized Experiments : Jove

|June 6, 2012

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This study standardizes pancreas dissection for organ donors, detailing regional islet cell variations and developmental origins. The procedure ensures comprehensive tissue sampling for diabetes research.

Area of Science:

Endocrinology
Anatomy
Developmental Biology

Background:

Standardized protocols are crucial for human pancreas tissue analysis in research programs like the Network for Pancreatic Organ Donors with Diabetes (nPOD).
Understanding regional islet cell distribution and developmental origins is key to interpreting pancreatic function and disease.
Previous studies highlight differences between human and rodent islets and regional variations within the human pancreas.

Purpose of the Study:

To establish a standardized dissection and sampling procedure for human pancreas from cadaveric organ donors.
To characterize the regional heterogeneity of islet composition, size, and number within the human pancreas.
To provide a comprehensive method for preserving tissue integrity for DNA, RNA, and protein isolation.

Main Methods:

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Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Published on: May 26, 2009

Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

Published on: July 30, 2011

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Last Updated: May 21, 2026

Collection Protocol for Human Pancreas

Collection Protocol for Human Pancreas

Published on: May 23, 2012

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Published on: May 26, 2009

Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

Published on: July 30, 2011

Developed a standardized dissection protocol involving dividing the pancreas into head, body, and tail regions.
Employed serial transverse sectioning and alternating sample allocation for fixed paraffin, fresh frozen, and snap-frozen preparations.
Included protocols for collecting associated tissues such as lymph nodes, spleen, and duodenum.

Main Results:

Demonstrated a standardized method for whole-pancreas sampling while maintaining anatomical orientation.
Characterized significant regional differences in islet composition, with the uncinate region rich in pancreatic polypeptide (PP) cells and other regions predominantly containing insulin (β)-cells.
Observed higher islet density in the tail region and noted potential atrophy in Type 1 Diabetes.

Conclusions:

The developed procedure effectively standardizes pancreas dissection and sampling for research purposes.
Revealed significant regional variations in human islet composition, linked to embryonic developmental origins.
This standardized approach supports comprehensive molecular analysis of pancreatic tissues in diabetes research.