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Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
09:04

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

Detection and characterisation of alloreactive T cells

Mandvi Bharadwaj1, Nicole A Mifsud, James McCluskey

  • 1Department of Microbiology and Immunology, University of Melbourne, Parkville, VIC, Australia. mandvi@unimelb.edu.au

Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2012
PubMed

T cell alloreactivity is responsible for much of the morbidity and mortality associated with tissue transplantation and graft versus host disease. Immunoassays for ex vivo monitoring and quantitation of alloreactive T cells are being increasingly utilised to provide valuable information for individualised clinical management of transplant recipients. Here we describe detailed methodologies for both traditional and novel assays utilised for the detection, quantitation, and functional characterisation of alloreactive T cells and highlight the key advantages and disadvantages of each system.

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