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Updated: May 21, 2026

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

Detection of allo-HLA cross-reactivity by virus-specific memory T-cell clones using single HLA-transfected K562 cells

Lloyd J D'Orsogna1, Ellen M W van der Meer-Prins, Yvonne M Zoet

  • 1Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre, Leiden, The Netherlands. Lloyd.DOrsogna@health.wa.gov.au

Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2012
PubMed

The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using single cell sorting apparatus, permitting the determination of alloreactivity from a single TCR with known specificity. When combined with new target "detector" cells called single HLA antigen-transfected K562 cells (SALs), the human alloresponse can for the first time be examined specifically and reliably. Here we describe a method for detection of "heterologous immunity" from virus-specific memory T-cells using single HLA expressing cell lines as allogeneic targets.

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