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Updated: May 21, 2026

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
Published on: March 6, 2019
Killer cell immunoglobulin-like receptors (KIR) typing by DNA sequencing
Lihua Hou1, Minghua Chen, Noriko Steiner
1Department of Pediatrics, CW Bill Young Marrow Donor Recruitment and Research Program, Georgetown University Medical Centre, Washington, DC, USA.
Abstract:
DNA sequencing is a powerful technique for identifying allelic variation within the natural killer cell immunoglobulin-like receptor genes. Because of the relatively large size of the KIR genes, each locus is amplified in two or more overlapping segments. Sanger sequencing of each gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.
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