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Updated: May 21, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
Roche genome sequencer FLX based high-throughput sequencing of ancient DNA
David E Alquezar-Planas1, Sarah L Fordyce
1Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, Denmark. dalquezar@snm.ku.dk
Abstract:
Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure that the data produced is optimal. Although much of the procedure can be followed directly from the manufacturer's protocols, the key differences lie in the library preparation steps. This chapter presents an optimized protocol for the sequencing of fossil remains and museum specimens, commonly referred to as "ancient DNA," using the Roche GS FLX 454 platform.
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