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Related Experiment Video

Updated: May 21, 2026

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Isotope-coded protein label.

Josef Kellermann1, Friedrich Lottspeich

  • 1Max-Planck Institute of Biochemistry, Martinsried, Germany. kellerma@biochem.mpg.de

Methods in Molecular Biology (Clifton, N.J.)
|June 6, 2012
PubMed
Summary
This summary is machine-generated.

We developed isotope-coded protein label (ICPL), a high-throughput method for quantitative proteome profiling. This technique accurately quantifies proteins in complex mixtures using mass spectrometry, aiding in biomarker discovery.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Stable isotope labeling coupled with mass spectrometry is crucial for protein identification and relative quantification in complex biological samples.
  • Existing methods face challenges in high-throughput quantitative proteome profiling on a global scale.

Purpose of the Study:

  • To introduce and validate a novel high-throughput quantitative proteome profiling method called isotope-coded protein label (ICPL).
  • To demonstrate the applicability of ICPL across diverse protein samples and its compatibility with standard proteomic workflows.

Main Methods:

  • ICPL involves tagging free amino groups of intact proteins with stable isotope derivatives.
  • Protein complexity is reduced using established separation techniques, followed by enzymatic cleavage.
  • Peptide ratios are determined via mass spectrometry (MS), with differential expression analysis using tandem MS for protein identification.

Main Results:

  • ICPL enables accurate and reproducible protein quantification with high sequence coverage.
  • The method is versatile, applicable to various protein samples like tissue and body fluid extracts.
  • A dedicated software, ICPLQuant, facilitates the quantification process for multiplexed experiments.

Conclusions:

  • ICPL offers a robust platform for global proteome profiling and quantitative analysis.
  • The method is essential for comprehensive detection of posttranslational modifications and distinguishing protein isoforms.
  • ICPL significantly advances high-throughput quantitative proteomics for biological and clinical research.