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Updated: May 21, 2026

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Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Published on: December 9, 2016
Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
Michal Domanski1, Kelly Molloy, Hua Jiang
1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark.
Biotechniques
|June 7, 2012
Abstract:
An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.

