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Updated: May 21, 2026
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A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans
Published on: March 14, 2019
Caenorhabditis elegans as a model in developmental toxicology
Windy A Boyd1, Marjolein V Smith, Jonathan H Freedman
1Biomolecular Screening Branch, National Toxicology Program, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 7, 2012
Summary
This study introduces a new Caenorhabditis elegans (C. elegans) assay for high-throughput screening of developmental toxicants. The assay utilizes the COPAS Biosort flow cytometer for efficient larval growth and development analysis.
Area of Science:
- Developmental Biology
- Toxicology
- Model Organisms
Background:
- Caenorhabditis elegans (C. elegans) is a valuable model organism in genetic and developmental research.
- C. elegans is increasingly used in toxicology due to conserved stress and disease pathways and available genetic resources.
- Current toxicological studies in C. elegans primarily focus on embryonic and larval growth tests.
Purpose of the Study:
- To describe a novel C. elegans larval growth and development assay.
- To adapt the assay for medium- and high-throughput screening.
- To detail data collection and analysis methods for the assay.
Main Methods:
- Utilized the COPAS Biosort flow cytometer for C. elegans analysis.
- Developed a larval growth and development assay.
- Implemented data collection and subsequent analysis protocols.
Main Results:
- The assay enables efficient screening of compounds affecting C. elegans development.
- The COPAS Biosort facilitates medium- and high-throughput data acquisition.
- Detailed data and analysis methods are provided for reproducibility.
Conclusions:
- The described assay enhances the utility of C. elegans in developmental toxicology.
- This method supports large-scale screening for toxicological compounds.
- The assay provides a robust platform for studying chemical impacts on nematode development.
