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Updated: May 21, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Methods for the determination of plasma or tissue glutathione levels
Trent E Tipple1, Lynette K Rogers
1Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA. tipple.10@osu.edu
Abstract:
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts are limited. Detailed instructions for performing each method as well as the advantages and disadvantages of each are discussed in this chapter.

