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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: May 21, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

HomozygosityMapper2012--bridging the gap between homozygosity mapping and deep sequencing.

Dominik Seelow¹, Markus Schuelke

¹NeuroCure Clinical Research Centre, Charité - Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany. dominik.seelow@charite.de

Nucleic Acids Research

|June 7, 2012

Summary

HomozygosityMapper is a free web tool that simplifies homozygosity mapping for recessive traits. This updated version supports Next Generation Sequencing data and diverse species, aiding genetic research.

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Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

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Last Updated: May 21, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

Published on: August 12, 2019

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

Area of Science:

Genetics
Bioinformatics

Background:

Homozygosity mapping is crucial for identifying recessive traits in consanguineous families.
Existing tools may lack flexibility in data input and analysis scope.

Purpose of the Study:

To introduce an enhanced version of HomozygosityMapper, a web-based tool for homozygosity mapping.
To improve the analysis of genetic data for identifying disease-related genes.

Main Methods:

HomozygosityMapper accepts genotype and deep sequencing data (including *.vcf files).
It identifies shared homozygous regions in affected individuals and integrates with the GeneDistiller candidate gene search engine.
New features include support for rodents and farm animals, extended analysis options, and exportable files for targeted sequencing and linkage analysis.

Main Results:

The new HomozygosityMapper version efficiently processes various data types, including Next Generation Sequencing data.
It offers expanded species support and refined analysis capabilities for single families and loss of heterozygosity studies.
The tool facilitates the generation of targeted enrichment and linkage analysis files, streamlining downstream research.

Conclusions:

HomozygosityMapper provides a comprehensive and user-friendly platform for homozygosity mapping.
The updated version enhances genetic analysis by supporting diverse data and species, and offering advanced features for gene discovery.