Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tight Junctions01:29

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
Alveoli and Alveolar Ducts01:26

Alveoli and Alveolar Ducts

The respiratory zone of the human body, which stands in contrast to the conducting zone, comprises the structures that actively participate in the exchange of gases. The initiation of this zone is marked by the terminal bronchioles converging into respiratory bronchioles, the tiniest bronchiole classification. The respiratory bronchioles give way to the alveolar ducts that opens into a congregation of alveoli. Actively involved in gas exchange, alveoli resemble tiny sacs similar to clusters of...
Transcellular Transport of Solutes01:23

Transcellular Transport of Solutes

Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...
Breathing01:05

Breathing

The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two muscle groups with important roles in breathing are the diaphragm, located directly below the lungs, and the intercostal muscles, which lie between the ribs. When the diaphragm contracts, it moves downward, increasing the volume of the thoracic cavity and creating more room for the lungs to expand. When the intercostal muscles contract, the ribs...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.

International journal of molecular sciences·2026
Same author

Claudin 4 Deletion Improves Gut Permeability and Survival in a Murine Model of Abdominal Sepsis.

Shock (Augusta, Ga.)·2026
Same author

Pioglitazone Modulates p65-Mediated Mitochondrial Bioenergetics: Implications for Acetaldehyde-Induced HIV Replication in Alveolar Macrophages.

Biomolecules·2025
Same author

Barriers to the Pharmacologic Rescue of W1282X CFTR.

Biochemistry·2025
Same author

Hyperglycemia differentially affects neutrophil transmigration across cystic fibrosis and wildtype bronchial epithelia.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2025
Same author

Impact of Endothelial Diversity and Dysfunction on Cardiovascular Disease.

Comprehensive Physiology·2025

Related Experiment Video

Updated: May 21, 2026

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses
10:30

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses

Published on: May 31, 2024

Roles for claudins in alveolar epithelial barrier function.

Christian E Overgaard1, Leslie A Mitchell, Michael Koval

  • 1Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory Alcohol and Lung Biology Center, Emory University, Atlanta, Georgia, USA.

Annals of the New York Academy of Sciences
|June 8, 2012
PubMed
Summary

Alveolar claudins (cldn) regulate lung fluid balance. While some claudins increase with lung injury, claudin-4 plays a protective role in maintaining alveolar barrier function.

More Related Videos

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
14:48

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Published on: December 26, 2012

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium
07:40

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

Published on: February 21, 2025

Related Experiment Videos

Last Updated: May 21, 2026

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses
10:30

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses

Published on: May 31, 2024

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
14:48

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Published on: December 26, 2012

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium
07:40

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

Published on: February 21, 2025

Area of Science:

  • Pulmonary Medicine
  • Cell Biology
  • Molecular Biology

Background:

  • The alveolar epithelium forms a critical barrier for gas exchange, maintaining lung fluid balance.
  • This barrier's integrity relies on tight junctions, regulated by claudin (cldn) proteins.
  • Alveolar claudins, including cldn-3, -4, -18, -5, and -7, are crucial for lung function.

Purpose of the Study:

  • To investigate the dynamic regulation of alveolar claudins in response to lung injury.
  • To understand the specific roles of different claudins in maintaining alveolar barrier function and fluid homeostasis.

Main Methods:

  • Analysis of alveolar claudin expression patterns in lung tissues.
  • Examination of the functional impact of claudin alterations on alveolar permeability and fluid clearance.

Main Results:

  • Lung diseases like alcoholic lung syndrome and acute lung injury alter alveolar claudin expression.
  • These alterations are often linked to impaired fluid clearance and increased alveolar leak.
  • Increased expression of claudin-4 demonstrates a protective effect on the alveolar barrier post-injury.

Conclusions:

  • Alveolar claudins are dynamically regulated, adapting lung barrier function to environmental and injury-related challenges.
  • Claudin-4 emerges as a key player in protecting alveolar integrity during lung injury.
  • Understanding claudin dynamics is essential for managing lung diseases characterized by fluid imbalance.