Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Redox Reactions

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...

Redox Reactions

Redox Reactions

Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...

Redox Equilibria: Overview

Redox Equilibria: Overview

A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...

Redox Titration: Overview

Redox Titration: Overview

Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...

Redox Titration: Other Oxidizing and Reducing Agents

Redox Titration: Other Oxidizing and Reducing Agents

Besides iodine, other oxidizing or reducing agents can serve as titrants in redox titrations. Common oxidizing titrants include KMnO4, cerium(IV), and K2Cr2O7. The choice of oxidizing titrants depends on factors like stability, cost, analyte strength, and reaction rate between the analyte and titrant. KMnO4 is a strong oxidizing titrant that reduces from Mn(VII) to Mn(II) in a highly acidic solution, simultaneously oxidizing the analyte to a higher oxidation state. In this case, KMnO4 acts as a...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glioblastoma-derived extracellular vesicles released after radiation promote cognitive impairment through NFκB-mediated microglial activation.

bioRxiv : the preprint server for biology·2026

Same author

An Artifact-Free Assay for the GSH/GSSG Ratio Adapted for Finger-Stick Blood Microvolumes: Simple, Sensitive, and Suitable for Any Laboratory.

Antioxidants (Basel, Switzerland)·2026

Same author

Peripheral immunochemical considerations in Parkinson disease: sources, targets and crosstalk mechanisms.

Journal of translational medicine·2026

Same author

GDF-11, GDF-15, Jag-1, and leptin in neuronal-derived extracellular vesicles as aging-related biomarkers to identify individuals at risk of Alzheimer's dementia: A pilot study.

Neurobiology of disease·2026

Same author

Proteome Signature of Alzheimer-Like Phenotypes in Frontal Cortices From Young and Old Individuals With Down Syndrome.

Molecular neurobiology·2025

Same author

Effects of cigarette smoke extract on surfactant production in ATII-like cells, involvement of oxidative stress and autophagy.

Toxicology·2025

Search research articles

Related Experiment Video

Updated: May 21, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

Redox proteomics.

D Allan Butterfield, Isabella Dalle-Donne

Antioxidants & Redox Signaling

|June 8, 2012

Summary

This summary is machine-generated.

Redox proteomics identifies protein oxidation changes linked to diseases and aging. This field uses advanced techniques to understand oxidative damage mechanisms and discover disease biomarkers.

More Related Videos

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

Related Experiment Videos

Last Updated: May 21, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

Cellular Redox Profiling Using High-content Microscopy

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

Area of Science:

Biochemistry and Molecular Biology
Proteomics and Mass Spectrometry

Background:

Proteins are susceptible to modifications by reactive oxygen and nitrogen species (ROS/RNS), altering their structure and function.
Redox proteomics studies these modifications to understand redox signaling and oxidative stress.
Perturbations in redox homeostasis are implicated in numerous diseases and aging processes.

Discussion:

Elucidating the cause-and-effect relationship between protein oxidation and human diseases is crucial.
Advanced methodologies combining proteomics, mass spectrometry (MS), and affinity chemistry are enhancing the understanding of protein oxidative modifications.
This field holds significant potential for identifying specific targets of oxidative damage.

Key Insights:

Redox proteomics offers powerful tools to investigate molecular mechanisms underlying diseases.
Studies cover diverse areas including neurodegenerative diseases (Alzheimer's, Parkinson's), chronic kidney disease, aging, and cardiovascular conditions.
The field is pivotal for discovering novel biomarkers for human diseases.

Outlook:

Continued advancements in redox proteomics are expected to deepen our understanding of disease pathogenesis.
Further research will focus on translating findings into clinical applications, such as diagnostics and therapeutics.
The integration of multi-omics approaches will likely accelerate discoveries in redox biology and disease.