Related Experiment Video
Updated: May 21, 2026
08:59
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
Racial differences in B cell receptor signaling pathway activation
Diane M Longo1, Brent Louie, Kavita Mathi
1Nodality, South San Francisco, CA 94080, USA. diane.longo@nodality.com
Journal of Translational Medicine
|June 8, 2012
Summary
African Americans exhibit distinct B cell receptor signaling compared to European Americans, with lower pathway activation linked to B cell frequency. This highlights race-associated differences in immune cell function.
Area of Science:
- Immunology
- Cell Signaling
- Flow Cytometry
Background:
- Single-cell network profiling (SCNP) measures intracellular signaling in immune cells.
- Previous SCNP identified race-associated differences in B cell PI3K pathway activity.
Purpose of the Study:
- To investigate race-associated differences in B cell receptor (BCR) signaling pathways.
- To extend SCNP analysis to multiple BCR signaling nodes in different racial groups.
Main Methods:
- SCNP was used to measure seven BCR signaling nodes in PBMCs from African American and European American donors.
- Analysis included multiple time points to assess signaling kinetics.
Main Results:
- African Americans showed lower anti-IgD induced phosphorylation of BCR pathway components (Syk, SFK, S6, Akt, Erk, p38, NF-κB) compared to European Americans.
- Racial differences were observed in both the magnitude and kinetics of BCR signaling.
- Frequency of IgD+ B cells differed by race and correlated with BCR pathway activation.
Conclusions:
- SCNP identified significant race-associated differences in BCR pathway activation in PBMCs.
- Understanding these racial contrasts in immune signaling is crucial for studying immune-mediated diseases.
More Related Videos
11:06Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
Published on: September 20, 2017
06:05A BW Reporter System for Studying Receptor-Ligand Interactions
Published on: January 7, 2019
Related Concept Videos
01:28Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
01:24B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:22Diversity in Cell Signaling Responses
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...
Graded and Abrupt Responses
Some signaling systems generate...
01:15Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...