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Related Concept Videos

ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and are...
ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
pH Regulation in Cells01:28

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

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Related Experiment Video

Updated: May 21, 2026

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
12:48

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

pH regulators in invadosomal functioning: proton delivery for matrix tasting.

Lucie Brisson1, Stephan J Reshkin, Jacques Goré

  • 1Nutrition, Growth and Cancer, Université François-Rabelais de Tours, Inserm U, France.

European Journal of Cell Biology
|June 8, 2012
PubMed
Summary

Invadosomes, crucial for cell-matrix interaction and remodeling, are vital in both normal physiological processes and aggressive cancer metastasis. This review highlights the role of pH regulators in invadosome function during bone resorption and cancer invasion.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Oncology

Background:

  • Invadosomes are actin-rich structures involved in extracellular matrix (ECM) remodeling.
  • They are found in normal cells (leukocytes, osteoclasts) and aggressive cancer cells, facilitating tissue invasion.
  • Invadosomes are classified into podosomes (normal cells) and invadopodia (cancer cells), sharing roles in adhesion and matrix degradation.

Purpose of the Study:

  • To review the critical role of pH regulators in invadosome function.
  • To emphasize the involvement of invadosomes in both physiological and pathological processes.
  • To focus on ECM remodeling by osteoclasts and cancer cells.

Main Methods:

  • Literature review focusing on invadosome structure, function, and molecular composition.
  • Analysis of the role of proteases and pH regulators in invadosome activity.
  • Examination of invadosome function in bone resorption and cancer metastasis.

Main Results:

  • Invadosome function relies on the recruitment and activation of proteases within an acidic microenvironment.
  • pH regulators, including V-ATPases and Na(+)/H(+) exchangers, are essential for invadosome formation and function.
  • Invadosomes are key mediators of ECM remodeling in bone resorption and cancer cell invasion.

Conclusions:

  • pH regulators are integral to invadosome-mediated ECM remodeling.
  • Understanding invadosome function and pH regulation is crucial for addressing diseases like osteopetrosis and cancer metastasis.