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Updated: May 21, 2026

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Published on: March 10, 2017

Putative link between Staphylococcus aureus bacteriophage serotype and community association.

D H Mohamed¹, S Saberesheikh, A M Kearns

¹Microbiology Services Division, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK. Mhadeq@yahoo.com

International Journal of Medical Microbiology : IJMM

|June 8, 2012

Summary

Community-associated Methicillin-resistant Staphylococcus aureus (CA-MRSA) can be distinguished from other MRSA strains by specific bacteriophage carriage. This finding aids in tracking MRSA epidemiology and understanding its virulence.

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Area of Science:

Microbiology
Genomics
Epidemiology

Background:

Methicillin-resistant Staphylococcus aureus (MRSA) is categorized into healthcare-associated (HA), community-associated (CA), and livestock-associated (LA) strains.
Genomic sequencing reveals variations in accessory components influencing MRSA pathogenicity and epidemicity.
Temperate bacteriophages are significant contributors to bacterial pathogenesis.

Purpose of the Study:

To develop and apply a microarray for identifying virulence-associated genes in Staphylococcus aureus.
To compare epidemic, pandemic, and sporadic lineages of UK HA-MRSA and CA-MRSA.
To investigate the correlation between specific bacteriophage serogroups and MRSA classification.

Main Methods:

Development and application of a partial composite Staphylococcus aureus virulence-associated gene microarray.
Comparative analysis of UK HA-MRSA and CA-MRSA isolates.
In silico analysis of identified phage structural genes and their correlation with phage serogroups.

Main Results:

Phage structural genes associated with CA isolates were identified.
CA strains predominantly carried Panton-Valentine leukocidin (PVL)-associated phages of serogroup A or Fb.
HA strains predominantly carried phages of serogroup Fa or B.

Conclusions:

The presence of specific PVL-associated bacteriophage serogroups (A/Fb) is correlated with community-associated MRSA status.
This correlation may be more indicative of CA status than the presence of luk-PV genes alone.
Findings contribute to understanding MRSA epidemiology and strain differentiation.