Enhanced biological responses of a hydroxyapatite/TiO2 hybrid structure when surface electric charge is controlled...

We investigated whether surface roughness and control of surface electric charge of a hydroxyapatite (HA)/titanium oxide (TiO2) hybrid coating could enhance biological responses associated with bone formation. After acid etching, a titanium surface was modified with HA and TiO2 by the dual sputtering deposition technique using radiofrequency sputtering. These surfaces were analyzed for surface roughness and surface electric charge intensity. Rat bone marrow-derived osteoblast-like cells were cultured on HA/TiO2 hybrid surfaces with different electric charges. The attachment and spreading behavior of these cells were significantly increased on the hybrid surface (p<0.05). In vivo experiment, the strength of bone-titanium implant integration with a hybrid surface was 3 times that of a control (p<0.05). The dual sputtering deposition technique created a HA/TiO2 hybrid structure. Our results show that the surface electric charge on a titanium surface is an important factor for enhancing biological responses.