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Retrospective analysis of sheep scrapie by western blotting with formalin-fixed paraffin-embedded (FFPE) tissues

Gantsetseg Dorj1, Hiroyuki Okada, Kohtaro Miyazawa

  • 1Prion Disease Research Center, National Institute of Animal Health, Tsukuba 305-0856, Japan.

The Journal of Veterinary Medical Science
|June 8, 2012
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.