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Related Experiment Video

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Diffusion Imaging in the Rat Cervical Spinal Cord
10:46

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Published on: April 7, 2015

In vitro and in vivo repeatability of abdominal diffusion-weighted MRI.

M E Miquel1, A D Scott, N D Macdougall

  • 1Clinical Physics, Barts Health NHS Trust, London, UK. m.e.miquel@qmul.ac.uk

The British Journal of Radiology
|June 8, 2012
PubMed
Summary

Diffusion-weighted MRI (DW MRI) shows good in vitro repeatability for apparent diffusion coefficient (ADC) measurements. In vivo abdominal measurements exhibit higher variability, requiring larger changes to be statistically significant, especially when using regions of interest (ROIs).

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Area of Science:

  • Radiology
  • Medical Imaging
  • Biophysics

Background:

  • Diffusion-weighted magnetic resonance imaging (DW MRI) is a valuable technique for assessing tissue microstructure.
  • Apparent diffusion coefficient (ADC) measurements are sensitive to cellular changes but can be affected by variability.
  • Understanding measurement variability is crucial for accurate interpretation of DW MRI findings in abdominal organs.

Purpose of the Study:

  • To evaluate the in vitro and in vivo variability of apparent diffusion coefficient (ADC) measurements.
  • To assess the impact of different regions of interest (ROIs) and volumes of interest (VOIs) on measurement reproducibility.
  • To determine the threshold for statistically significant changes in abdominal ADC values.

Main Methods:

  • DW MRI was performed using a multislice spin-echo echo-planar imaging sequence at 1.5 T.
  • In vitro studies utilized a flood-field phantom imaged over 100 days.
  • In vivo studies involved 10 healthy volunteers imaged on two separate occasions, with analysis of liver, spleen, kidney, and gallbladder using ROIs and VOIs.

Main Results:

  • Excellent in vitro repeatability was observed, with coefficients of variation below 1.3%.
  • No significant differences in group mean ADC values were found between in vivo visits for any organ.
  • In vivo reproducibility varied by organ and method: ROI-based reproducibility ranged from 20.0% to 28.0%, while VOI-based reproducibility improved significantly to 6.4%-9.6%.

Conclusions:

  • Good in vitro ADC measurement repeatability supports its reliability for phantom studies.
  • In vivo abdominal ADC measurements show higher variability, necessitating careful consideration of ROI/VOI selection.
  • Statistically significant changes in abdominal ADC require a >23.1% change with 2D ROIs but only >7.9% with large 3D VOIs.