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Gene mapping of mouse laminin A and B2 subunits using mouse-Chinese hamster somatic cell hybrids
N W Kaye1, A E Chung, P A Lalley
1Department of Anatomy, Cell Biology and Neurobiology, Albany Medical College, New York.
Somatic Cell and Molecular Genetics
|November 1, 1990
Abstract:
Laminin is a multichain extracellular matrix glycoprotein found primarily in basement membranes. The molecule is made up of three subunits, designated as A, B1, and B2. Using an 850-base cDNA probe for the mouse laminin A-chain and a 1000-base cDNA probe for the mouse laminin B2 chain, we have screened mouse-Chinese hamster somatic cell hybrids in order to assign the genes for each of these polypeptides to their respective mouse chromosome. We have determined that the mouse laminin B2-chain gene is located on chromosome 1 (confirming this assignment) and the laminin A-chain gene is located on chromosome 17.