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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

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Highly discriminatory typing method for Listeria monocytogenes using polymorphic tandem repeat regions.

Satoko Miya¹, Hajime Takahashi, Chikako Kamimura

¹Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan.

Journal of Microbiological Methods

|June 9, 2012

Summary

A new multilocus tandem repeat sequence analysis (MLTSA) method for Listeria monocytogenes offers higher discriminatory power than existing methods. This approach effectively distinguishes epidemic clones and aids in epidemiological studies of this foodborne pathogen.

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Area of Science:

Microbiology
Genetics
Epidemiology

Background:

Tandem repeats (TR) are polymorphic DNA sequences.
Listeria monocytogenes is a significant foodborne pathogen.

Purpose of the Study:

To develop a novel multilocus tandem repeat sequence analysis (MLTSA) method for Listeria monocytogenes typing.
To evaluate the discriminatory power and epidemiological utility of MLTSA compared to existing methods.

Main Methods:

Developed MLTSA using sequence polymorphisms in three tandem repeat regions.
Compared MLTSA results with a virulence-gene-based multilocus sequence typing (MLST) method.
Constructed dendrograms to cluster Listeria monocytogenes strains.

Main Results:

MLTSA successfully clustered strains into lineage I and lineage II.
Identified three distinct groups within lineage I, corresponding to major epidemic clones (ECI, ECIa, ECII).
MLTSA demonstrated higher discriminatory power than MLST and grouped some epidemiologically related isolates missed by MLST.

Conclusions:

MLTSA is a valuable tool for Listeria monocytogenes epidemiological studies.
The method offers improved strain discrimination and clustering capabilities.
Sequence polymorphisms in TR regions provide robust markers for pathogen typing.