Related Experiment Video
Updated: May 21, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Levels of non enzymatic antioxidants in Down syndrome
S Mehar Sulthana1, S Nandha Kumar, M G Sridhar
1Cytogenetics Division, Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry, India.
Objectives:
To confirm the clinical diagnosis of Down syndrome by chromosomal analysis and to explore the oxidative stress in children with Down syndrome by estimating the levels of non enzymatic antioxidants like reduced glutathione(GSH) and total antioxidants status (TAS).
Methods:
The study included 31 clinically diagnosed children with Down syndrome with equal number of age and sex matched controls. Trisomy 21 was confirmed by conventional lymphocyte cell culture. Erythrocytic reduced glutathione (GSH) and plasma total antioxidant status (TAS) were measured sphectrophotometrically.
Results:
The levels of erythrocytic reduced glutathione (GSH) and plasma total antioxidant status (TAS) were significantly reduced in children with Down syndrome.
Conclusions:
Children with Down syndrome have elevated levels of oxidative stress . Hence antioxidant therapy can be beneficial among them.
Related Concept Videos
Alzheimer Disease l: Introduction
Electron Transport Chain: Complex III and IV
Radical Autoxidation

