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Updated: May 21, 2026
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Isolation and analysis of small RNAs from virus-infected plants
Shaun J Curtin1, Agustín Zsögön, John M Watson
1Department of Agronomy and Plant Genetics, University of Minnesota, Minnesota, MN, USA.
Abstract:
In this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small RNAs (vsRNAs). These methods have been successfully used for four plant viruses: Cucumber mosaic virus (CMV), including the CMV Y-Satellite, Turnip mosaic virus (TuMV), Potato leaf roll virus (PLRV), and Tomato spotted wilt virus (TSWV) from inoculated Arabidopsis thaliana plants (Fusaro et al. EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA).
